Polymerase Chain Reaction.

A strip of eight PCR tubes, each containing a 100 μl reaction

Schematic drawing of the PCR cycle.

In this tiny tubes
I choose the gene of interest
That I want to amplify

We are the primers that had been design by
The Creator, Al-Khaliq

To complete this PCR process
We cannot be the same code
As we design to be on right and left
For forward and reverse reaction to occur
We pair up to complete each other
To help each other
The reaction will not occur
If both moving on the same direction.

We need the dNTP to form the chain,
As we need ilm’ to build up our life
We need the taq polymerase to generate the sequence,
As we need love to generate our path.
Buffer and water supply right environment for the reaction to occur,
As we need good background to build up our life

The recycling conditions;
Temperature and time to complete cycles
Play important role for PCR to success
Any mistake may lead to failure.
As we need to know how to control our temper
So that we will not lose anything

We aspect to obtain PCR product
With no mutation in it,
The gene of interest that had been amplified and purified
The gene that encode..

An old thermal cycler for PCR